#!/usr/bin/env python3 """tests for moog.py""" import os import random import re import string from subprocess import getstatusoutput from Bio import SeqIO from Bio.SeqUtils import GC from numpy import mean from itertools import chain prg = './moog.py' # -------------------------------------------------- def random_string(): """generate a random string""" return ''.join(random.choices(string.ascii_uppercase + string.digits, k=5)) # -------------------------------------------------- def test_exists(): """usage""" assert os.path.isfile(prg) # -------------------------------------------------- def test_usage(): """usage""" for flag in ['-h', '--help']: rv, out = getstatusoutput('{} {}'.format(prg, flag)) assert rv == 0 assert re.match("usage", out, re.IGNORECASE) # -------------------------------------------------- def test_bad_seqtype(): """die on bad seqtype""" bad = random_string() rv, out = getstatusoutput(f'{prg} -t {bad}') assert rv != 0 assert re.match('usage:', out, re.I) assert re.search( f"-t/--seqtype: invalid choice: '{bad}' \(choose from 'dna', 'rna'\)", out) # -------------------------------------------------- def test_bad_pctgc(): """die on bad pctgc""" bad = random.randint(1, 10) rv, out = getstatusoutput(f'{prg} -p {bad}') assert rv != 0 assert re.match('usage:', out, re.I) assert re.search(f'--pctgc "{float(bad)}" must be between 0 and 1', out) # -------------------------------------------------- def test_defaults(): """runs on good input""" out_file = 'out.fa' try: if os.path.isfile(out_file): os.remove(out_file) rv, out = getstatusoutput(prg) assert rv == 0 assert out == f'Done, wrote 10 DNA sequences to "{out_file}".' assert os.path.isfile(out_file) # correct number of seqs seqs = list(SeqIO.parse(out_file, 'fasta')) assert len(seqs) == 10 # the lengths are in the correct range seq_lens = list(map(lambda seq: len(seq.seq), seqs)) assert max(seq_lens) <= 75 assert min(seq_lens) >= 50 # bases are correct bases = ''.join( sorted( set(chain(map(lambda seq: ''.join(sorted(set(seq.seq))), seqs))))) assert bases == 'ACGT' # the pct GC is about right gc = list(map(lambda seq: GC(seq.seq) / 100, seqs)) assert .47 <= mean(gc) <= .53 finally: if os.path.isfile(out_file): os.remove(out_file) # -------------------------------------------------- def test_options(): """runs on good input""" out_file = random_string() + '.fasta' try: if os.path.isfile(out_file): os.remove(out_file) min_len = random.randint(50, 99) max_len = random.randint(100, 150) num_seqs = random.randint(100, 150) pct_gc = random.random() cmd = (f'{prg} -m {min_len} -x {max_len} -o {out_file} ' f'-n {num_seqs} -t rna -p {pct_gc:.02f} -s 1') rv, out = getstatusoutput(cmd) assert rv == 0 assert out == f'Done, wrote {num_seqs} RNA sequences to "{out_file}".' assert os.path.isfile(out_file) # correct number of seqs seqs = list(SeqIO.parse(out_file, 'fasta')) assert len(seqs) == num_seqs # the lengths are in the correct range seq_lens = list(map(lambda seq: len(seq.seq), seqs)) assert max(seq_lens) <= max_len assert min(seq_lens) >= min_len # bases are correct bases = ''.join( sorted( set(chain(map(lambda seq: ''.join(sorted(set(seq.seq))), seqs))))) assert bases == 'ACGU' # the pct GC is about right gc = list(map(lambda seq: GC(seq.seq) / 100, seqs)) assert pct_gc - .3 <= mean(gc) <= pct_gc + .3 finally: if os.path.isfile(out_file): os.remove(out_file)