diff --git a/.zuul.yaml b/.zuul.yaml
index 73085e3dff0..e2bcf196c0b 100644
--- a/.zuul.yaml
+++ b/.zuul.yaml
@@ -71,6 +71,29 @@
       bindep_profile: test py313
       tox_envlist: py313-threading
 
+- job:
+    name: nova-tox-functional-py313-threading
+    parent: openstack-tox-functional-py313
+    description: |
+      Run tox-based functional tests for the OpenStack Nova project
+      under cPython version 3.13 with eventlet disabled (native threading).
+      Uses tox with the ``functional-py313-threading`` environment.
+
+      This job also provides a parent for other projects to run the nova
+      functional tests with threading on their own changes.
+    required-projects:
+      # including nova here makes this job reusable by other projects
+      - openstack/nova
+      - openstack/placement
+    irrelevant-files: *functional-irrelevant-files
+    vars:
+      # explicitly stating the work dir makes this job reusable by other
+      # projects
+      zuul_work_dir: src/opendev.org/openstack/nova
+      bindep_profile: test py313
+      tox_envlist: functional-py313-threading
+    timeout: 7200
+
 - job:
     name: nova-tox-validate-backport
     parent: openstack-tox
@@ -950,6 +973,8 @@
             voting: false
         - nova-tox-functional-py310
         - nova-tox-functional-py313
+        - nova-tox-functional-py313-threading:
+            voting: false
         - tempest-integrated-compute:
            # NOTE(gmann): Policies changes do not need to run all the
            # integration test jobs. Running only tempest and grenade
@@ -979,6 +1004,8 @@
         - nova-live-migration
         - nova-tox-functional-py310
         - nova-tox-functional-py313
+        - nova-tox-functional-py313-threading:
+            voting: false
         - nova-multi-cell
         - nova-next
         - nova-tox-validate-backport
diff --git a/tox.ini b/tox.ini
index 3a6c56e56fc..be083025c7d 100644
--- a/tox.ini
+++ b/tox.ini
@@ -118,6 +118,27 @@ commands =
   stestr --test-path=./nova/tests/functional run {posargs}
   stestr slowest
 
+[testenv:functional-py313-threading]
+description =
+  Run functional tests with native threading (eventlet disabled).
+passenv =
+  {[testenv]passenv}
+  GENERATE_SAMPLES
+setenv =
+  {[testenv]setenv}
+# we do not have any greenlet leaks in functional tests so enforce that
+# by making greenlet leaks a failure.
+  NOVA_RAISE_ON_GREENLET_LEAK=True
+# run the test without eventlet (native threading)
+  OS_NOVA_DISABLE_EVENTLET_PATCHING=True
+deps =
+  {[testenv]deps}
+  openstack-placement>=9.0.0.0b1
+extras =
+commands =
+  stestr --test-path=./nova/tests/functional run {posargs}
+  stestr slowest
+
 [testenv:functional-without-sample-db-tests]
 description =
   Run functional tests by excluding the API|Notification